Intracytoplasmic Sperm injection (ICSI)

Intracytoplasmic Sperm injection (ICSI)

ICSI-Intra Cytoplasmic Sperm Injection is a technique by which a single sperm is injected into the egg to aid fertilisation process to make the embryo (fertilised egg).

There are 2 ways to fertilise the eggs in the laboratory. The conventional insemination-IVF in which the eggs and sperm are mixed in a petri dish and the sperm spontaneously enter the egg to bring about fertilisation. However, in certain situations such as low sperm count when normal fertilisation may not occur, ICSI is done to facilitate fertilisation.

Indications for ICSI

ICSI is offered in the following situations

  • Abnormally low sperm count
  • Sperm showing poor motility
  • Very high proportion of abnormal looking sperm
  • Previous failed/poor fertilisation with IVF
  • Sperm obtained from testis-surgical sperm retrieval
  • Unexplained subfertility
  • Very low number of eggs/poor quality eggs

How is the process of ICSI done?

When the eggs are retrieved, they are surrounded by supporting cells called cumulus. With ICSI, the embryologist removes these surrounding supporting cells by placing them in special solutions and then mechanically using special pipettes to isolate the egg.

The egg is then placed in customised dishes under the microscope of the micromanipulator-machine used to perform ICSI in special droplets. The egg is then held by a special holding pipette and a single sperm is then selected under the microscope by the scientist and loaded inside the injection pipette which is then introduced into the egg thus depositing the sperm inside the egg. We basically perform all the work for the sperm by depositing it inside the egg.

From the patient’s perspective, there are no changes in the protocol it is exactly the same as for IVF. In the lab, we use the micromanipulator to perform ICSI to facilitate fertilisation.

Sperm selection process for ICSI

The healthy sperms have an oval head, a good midpiece and a long tail for motility. In men with poor sperm quality, these healthy sperms are very few in number and hence it is important to select a structurally good sperm to achieve better results with fertilisation.

The sperm is prepared and placed in special dishes in viscous solutions to slow them down and then the most normal looking sperm is selected, immobilised using the glass pipette and then sucked into the injection pipette ready to be injected.

Our embryologists are experts in choosing the right sperm and sometimes spend hours to select normal looking sperm (samples obtained from testis where there is no ejaculated sperm) to perform ICSI.

At KIMS fertility centre, the team uses additional techniques such as IMSI (Intracytoplasmic morphologically selected sperm injection)  where necessary to select better sperm. Additional advanced techniques such as calcium to activate the egg and sperm activation agents are also used in certain situations to improve the outcome of ICSI and our embryologists have been trained in all the latest techniques to optimise the outcome for our patients undergoing ICSI.

ICSI provides a safe and effective way of fertilising the egg which would not have fertilised otherwise. Very little damage if any is incurred by the egg when performed by our very experienced embryology team who are veterans in this field. All the fertilised eggs are then placed in the incubator for further growth and development.

MotherToBe ICSI fertility center in Hyderabad recommends this next-level treatment in the case where the male patient has very low sperm counts or sperms don’t have sufficient motility or movement. ICSI is also proposed to patients whose sperms are abnormally shaped, in which fertilization is not possible even in the test tube. We provide world-class ICSI treatment in Hyderabad that brings high success rates with conventional IVF in most cases of male factor infertility.

Our successive ICSI fertility treatment in Hyderabad is also preferred in fertility preservation cases to ensure that we don’t have an unexpected fertilization failure, since most of the fertility preservation patients don’t have time to undergo a repeated cycle

 ICSI-Intracytoplasmic Sperm Injection is an advanced laboratory procedure that aids infertile couples undergoing IVF due to male factor infertility.  It is the revolutionized treatment for severe & otherwise non-treatable male infertility. Success rates with Intracytoplasmic Sperm Injection with IVF center and the skill of the fertility doctors. Undergoing ICSI fertility treatment in Hyderabad would potentially receive counseling and holistic fertility care.

To achieve fertilization, there should be enough motile sperm to bind & subsequently penetrate the egg. If the number of sperms are too low, then ICSI-Intracytoplasmic sperm injection is utilized. This decision to perform ISCI is made four to six hours after the egg retrieval.

MortherToBe ICSI fertility center in Hyderabad considers various factors that contribute to the decision to use ICSI include semen parameters such as sperm concentration (number of sperms per mm), motility, morphology (shape characteristics), the number of oocytes/eggs retrieved, and the presence/absence of anti-sperm antibodies and even the results from any previous IVF cycles.

ICSI Process in Detail

In the case of insemination at the ICSI fertility hospital in Hyderabad, the male partner’s sperm is processed to achieve an optimal concentration of fertilization of the eggs.  In the first step, the semen sample is washed with a special solution and then placed in a centrifuge to concentrate the sperm and separate them from the other components from the semen.

The sperm in the pellet is re-suspended and placed in more of the same special solution while the remaining special fluid is discarded. The eggs are left remained in the cumulus-complexes which is required for sperms to achieve the final activation steps. The whole process is done by the expertise fertility specialists or embryologists of the leading ICSI fertility clinic in Hyderabad.

At MotherToBe ICSI fertility hospital in Hyderabad believes that expertise combined with advanced lab equipment, and technology would surely increase the success rates of treatment. and the predetermined sperm is placed in the media drops that contain the oocytes approx 4-6 hours, after the egg retrieval. The eggs and sperms are then returned to the incubator for sixteen to seventeen hours.

If it is determined that Intracytoplasmic Sperm Injection-ICSI is the best method to accomplish fertilization, the cumulus complexes are removed from the eggs. Removing these allows embryologists and fertility specialists to visually determine which eggs have reached maturity and hence, have the capacity to get fertilized.

The removal of these outer cells is necessary for the embryologists to be able to technically perform ICSI at the best ICSI hospital in Hyderabad. Only mature eggs are injected with the sperm. While observing each mature egg under the microscope and a single yet most viable sperm is drawn into a needle and injected directly into the egg’s cytoplasm.

MotheToBe being the best ICSI clinic in Hyderabad, we have been helping many childless couples to fulfill the dream of having a healthy baby. Our doctors and embryology experts take on the toughest male factor infertility cases, providing them renewed hope for to-be parents.

Since MotherToBe ICSI clinic in Hyderabad opened its doors more than 20 years ago, we have been helping our patients and nearly created over 9500 miracles through IVF, IUI, ICSI, IMSI, egg donation, and various minimally invasive fertility surgeries. MotherToBe ICSI fertility center in Hyderabad has brought world-class care to infertile patients to embrace their baby dreams.

 Male factor infertility could be influenced by a wide range of medical issues. Thorough evaluation and treatment from a urologist with extensive expertise in this particular male infertility is critical. If you have any infertility concerns, Seek help from the top and the best ICSI fertility center in Hyderabad and schedule an appointment.


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Are the success rates better with ICSI than IVF?

The pregnancy rates are similar with IVF and ICSI. However, ICSI is a boon and has revolutionised the treatment of male infertility in that even men with few thousands of sperm or men with no sperm in semen (azoospermia) are also able to get their own genetic child using this technique.

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